Lycorine의 사람 구강 암 세포주에서 survivin 단백질 분해 증진으로 세포자멸사 유도
Lycorine induces apoptosis by enhancing protein degradation of survivin in human oral cancer cell lines
정요셉, 조남표, 장분실,
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정요셉 ( Jeong Joseph H. ) - 서울대학교 수의과대학 발육생유전학과
조남표 ( Cho Nam-Po ) - 전북대학교 치의학대학원 구강병리학교실
장분실 ( Jang Boon-Sil ) - 전북대학교 치의학대학원 구강병리학교실
KMID : 0829220170410010001
Abstract
Lycorine, a natural alkaloid extracted from the Amaryllidaceae plant family, was reported to various physiological and pharmacological effects including anti-cancer activity. Nevertheless, there is no report of the anticancer effect of lycorine in oral cancer cells. The effects of lycorine on cell proliferation and apoptosis were examined through trypan blue exclusion assay, 4’-6-diamidino-2-phenylindole (DAPI) stain, Live/Dead assay, Western blot analysis and RT-PCR. Lycorine suppressed cell viability and induced apoptosis in MC3 and HSC-3 cell lines. Lycorine decreased survivin protein but did not affect its mRNA. It regulated survivin through accelerating protein degradation in a time-dependent manner although neither proteasome nor lysosome was not associated with lycorine-mediated protein degradation. Collectively, our results suggest that lycorine may be a potential therapeutic anti-cancer drug candidate for the treatment of human oral cancer.
키워드
Lycorine; Apoptosis; Survivin; Human oral cancer
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